Apart from the diva software, the bd facscanto ii system includes a clinical software designed to address the needs of todays busy clinical laboratory. Collating data from global sites, data managing and uploading to the sponsor sites as per. Pdf accurate and reproducible enumeration of t, b, and nk. The facscanto ii offers unbeatable consistency and optimal signal detection with increased resolution and sensitivity for multicolor assays. Principles of flow cytometryprinciples of flow cytometry mohamed labib salem, phd prof. The bd facscanto ii system delivers flow cytometry data on an efficient, user friendly platform. Slyric system with bd facsuite clinical software, and on the bd facscantoii system. Bd biosciences is approved as a provider of continuing education programs in the clinical laboratory sciences by the ascls p. Figure 11 bd facscanto ii flow cytometer the bd facscanto ii flow. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility.
Analysis of flow cytometry patient data of clinical trials using bd facsdiva software. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. The bd facscanto family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters. The bd facscanto ii provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors. Home products manufacturers bd biosciences bd facscanto ii products. Jul 05, 20 facscalibur ii is a stalwart workhorse in the clinical and research lab with a 3 color, 1 laser blue system, or 4 color 2 laser blue, red system.
The development of seven and eightcolor assays on this device will require. The february 2020 security rollupcumulative update was released in february 2020. Bd facscanto ii canto 10color with canto clinical software. Its flexible, modular design offers automation and up to eightcolor capacity for rapid and easy production of highquality data in clinical. Manage symptoms from pleural effusions or malignant ascites at home. Using bd panels with bd facs spa and bd facscanto clinical software and the bd facslink lis interface. Jun 07, 2006 the bd facscanto is often hailed as the new generation of flow cytometry. Standardization of 8color flow cytometry across different flow cytometer instruments. Bd facsdiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the bd facscanto ii workstation. Use bd facscanto clinical software for performing cytometer quality control. New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software version, etc. Updated for 6color reagent and bd facscanto clinical software v2. Instrument optimization for bd facscanto instruments creating application settings for white blood cells using lysewash, or lyseno wash methods.
The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Contact us today at 408 5123901 for further details. One striking difference between the facscanto and older generation machines is that this system operates on a pcbased windows xp platform using bd facsdiva software. The bd bioscience facscanto ii flow cytometer is a benchtop analyzer providing reliable and accurate results for experiments with up to 10 parameters. Built on more than 30 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto flow cytometry system delivers reliable performance, accuracy, and easeofuse for todays busy clinical.
The bd facscanto ii system can optionally be used with the bd facs loader for automatic sample introduction, a standalone barcode reader for data input into bd facscanto clinical software, and with the bd facs sample prep assistant ii. Bd biosciences technical field service engineer can be. Bd biosciences recommends that firsttime users of this cytometer takeadvantage of. The optional bd facscanto ii handheld barcode reader reads twodimensional barcode labels and it is used to read the compensation matrix of the bd facs 7color setup beads reagent into bd facscanto clinical software. Bd facscanto ii quick reference manual pdf download. The optics of the bd facscanto ii system consist of an. The software uses an automatic gating algorithm to identify and isolate stem cells. The expert staff at nyu langones cytometry and cell sorting laboratory provides researchers with training on and access to a facscanto analyzer. Bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Flow cytometry patient data of clinical trials using bd facsdiva software. Bd biosciences recommends that firsttime users of this cytometer. Bd facs canto ii operators manual marshall scientific issuu. Our bd facscanto ii is a 3 laser red 633nm, blue 488nm and violet 405nm cytometer capable of analyzing up to 8 fluorescence parameters and 2 scatter parameters fsc and ssc.
View online or download bd facscanto ii instructions for use manual, quick reference manual. Bd bioscience facscanto ii flow cytometer marshall. Bd synapsys solution provides secure and scalable connectivity for bd bactec blood culture solution, bd kiestra lab automation solution and, in the u. The bd facscanto ii system uses a fixedoptics design and advanced digital. It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd facs. Chetna dwivedi clinical system verification engineer ii.
Facscanto ii is a clinical, as well as research, instrument with many automated controls, and generally comes as a 3 laser blue, red, violet, 8 color instrument. The ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical laboratory. See headers and footers on page 100 in the bd facsdiva software reference manual. Bd facs sample prep assistant iii accessory to the bd facscanto system with bd facscanto clinical software. Samples were analyzed on the bd facslyric system with bd facsuite clinical software, and on the bd facscanto ii system with bd facscanto clinical software and bd facs 7color. Win32k patch was released with december 2019 security updates, crypto api patch. Win32k patch was released with december 2019 security updates, crypto api patch was released with january 2020 security updates. The bd facscanto comes with bd facscanto clinical software and bd facsdiva software. Bd facscanto ii flow cytometer reference manual alfresco. Bd facscanto flow cytometer 433 configuration with bd facscanto clinical software and bd facsdiva software and bd facscanto ii flow. Application name bd facsdiva, bd facscanto clinical software. Software acquisition and analysis methods are based on the method featured in the clinical. Use bd facscanto clinical software for performing cytometer qualitycontrol.
Page 1 bd facscanto ii instructions for use for in vitro diagnostic use part no. Bd facscanto clinical software overview features workflow sample data productivity, consistency, and reliability from dedicated clinical software the ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical. Cell protein dna core facility georgia state university. A bd hlab27 software module for the bd facscanto ii system provides a complete system for rapid detection of hlab27 antigen, which is clinically relevant to the evaluation of seronegative spondyloarthropathies.
Bd bioscience facscanto ii flow cytometer marshall scientific. Built on more than 30 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto flow cytometry system delivers reliable performance, accuracy, and easeofuse for todays busy clinical laboratories. The bd facscanto ii system can optionally be used with the bd facs loader for automatic sample introduction, a standalone barcode reader for data input into bd facscanto clinical software, and with the bd facs sample prep assistant ii for automatic sample preparation of assays utilizing the lysenowash method. Jan 21, 2012 facscanto ii offers walkaway sample handling with the carousel loader 40 tubes capacity or high throughput sampler 96 or 384well plate loader. Bd facs canto ii operators manual marshall scientific. Stratedigm offers its flow cytometers as a superior alternative to the bd lsr ii and bd fortessa. The innovative designs for both the excitation and collection optics reduce excitation losses, yielding more information from each sample. As a leading provider of tools for flow cytometry, bd biosciences is committed to offering comprehensive, innovative software. Accurate and reproducible enumeration of t, b, and nk.
Bd facscanto ii flow cytometer quick reference guide. Bd facscanto ii becton dickinson, san jose, ca, usa. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. The bd facscanto ii is designed to address the needs of todays busy clinical lab. Bd facs canto ii flow cytometry system gmi trusted. It contains important information that is not included in the printed or electronic documentation. Cytometry bd facscanto ii laser errors happy new year to all. It includes automated setup and compensation, applicationspecific analysis modules, and predefined reports with integrated quality control. Flow cytometry analyser ivd, automated a mains electricity acpowered laboratory instrument intended to be used to count, examine andor sort cells or microscopic particles in a clinical specimen e. Built on more than 25 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto ii system is an easytouse benchtop analyzer that delivers proven performance, accuracy, and highquality results. Running samples with bd facscanto clinical software.
Bd biosciences recommends that firsttime users of this cytometer take advantage of operator training offered with. Bd facscanto clinical software is supplied with the bd facscanto and facscanto ii systems and includes application panels optimized for use with specific ivd reagent kits. At a time when health care systems around the globe are struggling to keep up with the influx of patients from the covid19 crisis, bd employees with clinical backgrounds are stepping up to help. Before you begin z stain either the high or the low bd stem cell control. Bd biosciences facscanto ii flow cytometer features optics. Bd synapsys solution allows connectivity for any number of supported instruments to one or more lis systems.
Bd facs canto ii operators manual marshall scientific by. Bd biosciences facscanto ii flow cytometer product list. Bd facscanto clinical software with spa ii sample prepper bd facscanto clinical software with spa iii sample prepper using bd panels with bd facscanto clinical software and the bd facslink lis interface. Applications are far reaching and can be found on the bd online tutorial. The bd facscanto ii is a powerful multicolour benchtop analyser offering easy to use, powerful and reliable performance.
Christopher sequeira ms, ccy technical training engineer. Bd facsdiva software provides a wide array of basic analysis features, enabling multiple applications to run on the bd facscanto, bd lsr, and bd facsaria cytometers. These tools assist in the ease and efficiency of using facscanto as they enable automated features. View christopher sequeira ms, ccys profile on linkedin, the worlds largest professional community. The pleurx system helps patients take control of uncomfortable and painful symptoms from pleural effusions or malignant ascites. Bd biosciences bd facscanto ii flow cytometers 422 and 53 configurations with bd facscanto clinical software and bd facsdiva software. It automatically sets cytometer detector voltages and creates compensation tables based on a single run with bd. Provides streamlined software workflows for acquisition and analysis.
Bd facscanto clinical software further automates setup, compensation analysis, and quality control for predefined clinical applications. It provides a high degree of automation and quality control to reduce hands on time for technicians and improve. Bd facsdiva software for setup, acquisition, and analysis bd facsdiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the bd facscanto ii workstation. System options include 8color 3laser system 405 488 633 nm 8color 2laser system 488 633 nm a 2d. Find a wide selection of bd biosciences facscanto systems in auctions. Saves time, improves reproducibility high performance, innovative system the bd facscanto ii fluidics cart is. Collating data from global sites, data managing and. Bd facsdiva software reference manual for version 8. Setup on page 1 in the bd facsdiva software reference manual. Sections are indicated with the appropriate software. Bd biosciences facscanto ii flow cytometer features.
Bd facsdiva operating software is common across many bd cell analyzers and cell sorters, including bd lsr and bd. Bd horizon brilliant polymer dyes are four to ten times brighter than conventional dyes, with equivalent backgrounda breakthrough in the field. Minimize, maximize, and close buttons in title bar minimize buttonreduces the application to a button on the windows taskbar. Multisite evaluation of the bd stem cell enumeration kit for. This software is for use only on the bd facscanto and bd facscanto ii. It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd facs 7color setup beads, applicationspecific analysis modules, and predefined reports with integrated quality control. Standardization of 8color flow cytometry across different. Daily routine procedures such as startup, shutdown, and cleaning routine cycles are automated as a result of fluidic integration with bd facscanto clinical software or bd facsdiva software. These panels feature automated gating, calculations, and report generation to deliver consistent, reproducible, and standardized analysis.
A june 2007 bd biosciences benex limited canada japan bay k 1 ad nippon becton. Bd facscanto ii is a flow cytometer intended for the qualitative and quantitative measurement of. Bd facscanto clinical software set up the cytometer with bd facscanto clinical software. A proven platform for high reliability and quality results. Bd facscanto fluidics with bd facscanto clinical software or bd facsdiva software. The bd facscanto ii provides the ultimate in flexibility and can be configured with two or three. Find out firsthand how the unique capabilities of the bd facsvia flow cytometry system are designed with clinical simplicity in mind for excellence in blood cell enumeration and analysis. A feasibility study in clinical laboratories in switzerland. Discover clinical simplicity with the bd facsvia flow. Bd facsdiva software pc performs sample acquisition 18 bits5 decades and data analysis. Instrument installation, operational, and perf clinical cytometry. Firsttime users of bd facsdiva software should read. Discover clinical simplicity with the bd facsvia flow cytometry system.
It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd. Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. Becton dickinson bd facscanto ii and beckman coulter bc navios, aiming at increasing reproducibility and interlaboratory comparability of immunophenotypic data in clinical. Bd facscanto ii becton dickinson, san jose, ca, usa data file structure.
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